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Biological concentrations of DDT metabolites and breast cancer risk: an updated systematic review and meta-analysis.

Ugalde-Resano et al,

2025

Rev Environ Health

This comprehensive systematic review and meta-analysis examined the relationship between exposure to DDT (a banned pesticide that persists in the environment and human body) and breast cancer by analyzing studies published from 2000 to 2021. The analysis found positive associations between DDT exposure and breast cancer risk across multiple study types: in long-term prospective studies, women with higher p,p’-DDT levels had 41% increased odds of breast cancer, while retrospective studies showed 15% increased odds with p,p’-DDE exposure and 33% increased odds with high p,p’-DDT concentrations. The strongest association was observed with o,p’-DDT exposure, showing 2.24 times higher odds of breast cancer. These findings support a positive relationship between DDT exposure and breast cancer risk, reinforcing the importance of maintaining the worldwide ban on DDT use, as this pesticide remains in human bodies for decades and continues to spread geographically even where it’s no longer applied.

Endocrine-disrupting chemicals: A Systematic Review of Epidemiological Studies

Wan et al,

2022

Crit Rev Food Sci Nutr

A systematic review of 131 epidemiological studies examining endocrine-disrupting chemicals (EDCs) and breast cancer risk found evidence that exposure to various EDCs—including pesticides (DDT/DDE, atrazine, dioxin), synthetic chemicals (BPA, phthalates, PFAS, PCBs, PBDEs), and other compounds found in everyday products—may elevate breast cancer risk, particularly when exposure occurs during early life. The review identified food as a major route of EDC exposure and emphasized that because most EDCs persist in the environment and accumulate in the body over time, long-term multi-generational health impacts need to be assessed. The authors call for improved exposure assessments of EDCs in food and food packaging, along with careful evaluation of their links to breast cancer development to inform policy-making and regulations aimed at protecting public health.

DDT and Breast Cancer: Prospective Study of Induction Time and Susceptibility Windows.

Cohn et al,

2019

J Natl Cancer Inst

This study of over 700 women found that DDT exposure was associated with increased breast cancer risk through age 54, but the timing of first exposure mattered critically: women first exposed to DDT after infancy had nearly triple the risk of early postmenopausal breast cancer (ages 50-54), while women first exposed during infancy through puberty had nearly four times the risk of premenopausal breast cancer (before age 50). These findings suggest there are specific windows of vulnerability when DDT exposure has the strongest impact on future breast cancer risk, with effects that can persist for decades. The results support the idea that DDT acts as an endocrine disruptor affecting breast tissue throughout a woman’s life—from before birth through menopause—even though DDT was banned in the U.S. in 1972, highlighting the long-term health consequences of early-life chemical exposures.

Plasma levels of dichlorodiphenyldichloroethene (DDE) and dichlorodiphenyltrichloroethane (DDT) and survival following breast cancer in the Carolina Breast Cancer Study.

Parada et al,

2019

Environ Int

This 20-year study of 748 women diagnosed with breast cancer in North Carolina found that those with the highest levels of DDE (a breakdown product of the banned pesticide DDT) in their blood had nearly twice the risk of death compared to those with the lowest levels. The association between DDE exposure and breast cancer death was particularly strong among Black women and women with estrogen receptor-negative tumors, with Black women showing more than double the mortality risk. Since DDT was banned in the U.S. in 1972 but DDE persists in the environment and body fat for decades, these findings suggest that legacy pesticide exposure may contribute to worse breast cancer outcomes and racial disparities in breast cancer survival.

Influence of exposure to endocrine disruptors and other environmental chemicals on breast development in girls: A systematic review of human studies.

Olivas-Martínez et al,

2025

Int J Hyg Environ Health

New research highlights the link between endocrine-disrupting chemicals (EDCs) and earlier breast development in girls. A systematic review of 68 studies found that 10 out of 14 high-quality studies linked prenatal and postnatal exposure to EDCs like organohalogenated compounds and phthalates to earlier thelarche. With thelarche now occurring nearly three months earlier per decade, these findings raise serious concerns about long-term health risks, including breast cancer. Reducing exposure to harmful chemicals in everyday products is crucial to protecting hormonal health and preventing early puberty.

Impact of DDT on women’s health in Bangladesh: escalating breast cancer risk and disturbing menstrual cycle.

Subah et al,

2024

Front Public Health

DDT, a highly persistent organochlorine pesticide comprising mainly p,p’-DDT (77%) and o,p’-DDT (15%), was widely used in agriculture and disease vector control with India being the primary consumer for malaria and leishmaniasis control, though global usage declined from ~5,388 metric tons annually (2001-2007) to ~3,772 metric tons (2008-2014) following the Stockholm Convention. As a xenoestrogen that bioaccumulates in fatty tissues and breast milk, DDT disrupts the endocrine system and enters the human circulatory system, causing reproductive toxicity, increased cancer risk (particularly threatening infants consuming contaminated breast milk), and carcinogenic effects, while also devastating wildlife populations through eggshell thinning in birds like pelicans and eagles. Prolonged DDT exposure causes cumulative toxicity that can alter morphogenesis, induce cancer, and cause reproductive system failure, yet its exceptional persistence and resistance to biodegradation means it continues to accumulate in the food chain and pose ongoing health threats despite reduced usage.

Endocrine-disrupting chemicals and breast cancer: a meta-analysis.

Liu et al,

2023

Frontiers

This meta-analysis aimed to evaluate the association between exposure to endocrine-disrupting chemicals (EDCs), including phthalates and other common environmental pollutants, and breast cancer risk. The study found that certain EDCs—such as p,p′-DDT, chlordane, HCH, and specific PCBs—were positively associated with increased breast cancer risk, while a few compounds like BBP and PFDoDA showed a negative association.

Endocrine disrupting chemicals and breast cancer: a systematic review of epidemiological studies.

Wan et al,

2022

Crit Rev Food Sci Nutr

This systematic review of 131 epidemiological studies evaluated the association between various endocrine-disrupting chemicals, including phthalates and hormonal exposures like contraceptive pills, and the risk of breast cancer. It found that several EDCs, particularly phthalates and oral contraceptive use, were consistently associated with increased breast cancer risk across multiple studies.

Exposure to organochlorine pesticides as a predictor to breast cancer: A case-control study among Ethiopian women.

Mekonen et al,

2021

PLOS One

A case-control study in Ethiopia examined 50 breast cancer patients and 50 controls to investigate whether exposure to organochlorine pesticides is a risk factor for breast cancer in a low- and middle-income country context. Ten organochlorine pesticides were detected in participants’ serum, with heptachlor found at highest concentrations; mean serum levels of p,p’-DDE, p,p’-DDT, heptachlor, gamma-chlordane, endosulfan, and dibutyl-chlorendate were significantly higher in breast cancer patients than controls, and p,p’-DDT and gamma-chlordane emerged as significant predictors of breast cancer, with each unit increase in p,p’-DDT concentration doubling breast cancer odds (AOR = 2.03; 95% CI: 1.04-3.97) and each unit increase in gamma-chlordane tripling the odds (AOR = 3.12; 95% CI: 1.19-8.20). These findings suggest that organochlorine pesticide exposure may be a significant breast cancer risk factor in Ethiopia, where environmental contamination from these persistent organic pollutants remains a concern despite global restrictions. The study highlights the public health importance of reducing exposure to these banned or restricted pesticides in developing countries and emphasizes the need for continuous biomonitoring of persistent organic pollutants to inform disease prevention strategies and mitigation measures, particularly as breast cancer incidence rises in low- and middle-income countries where environmental regulation may be less stringent.

Environmental exposures and breast cancer risk in the context of underlying susceptibility: A systematic review of the epidemiological literature.

Zeinomar et al,

2020

Environ Res

A systematic review of 100 publications across 56 epidemiologic studies found that research enriched with women at higher baseline breast cancer risk—through family history, early-onset disease, or genetic susceptibility—consistently showed stronger and more frequent associations between environmental chemical exposures and breast cancer compared to average-risk populations. Specifically, 80% of studies enriched with family history or early-onset cases showed significant associations with exposures including PAHs, air pollution, DDT, PCBs, PFAS, metals, personal care products, and occupational chemicals, while 74% of studies examining genetic susceptibility found significant gene-environment interactions for various pollutants in women with variants affecting carcinogen metabolism, DNA repair, and oxidative stress. These findings suggest that the inconsistent evidence for environmental chemicals and breast cancer in the literature may partly stem from studying predominantly average-risk populations who may be less susceptible to environmental carcinogens, highlighting the critical need for future research to focus on high-risk populations and measure exposures during key windows of susceptibility (puberty, pregnancy, menopause) to more accurately capture the role of environmental chemicals in breast cancer development.

Correlation of body mass index with serum DDTs predicts lower risk of breast cancer before the age of 50: prospective evidence in the Child Health and Development Studies.

Cohn et al,

2019

J Expo Sci Environ Epidemiol

This study from a longitudinal cohort of 133 women diagnosed with breast cancer before age 50 and 133 age-matched controls without breast cancer. DDT is stored in adipose tissue, and the authors found that serum DDE (the main metabolite of DDT) was inversely associated with BMI amont women who were cancer-free, but that this association did not hold among women diagnosed with breast cancer before age 50. The authors suggest that early exposure to breast cancer among women exposed to DDT may be due to an uncoupling of the relationship between BMI and serum DDT, and that this may reveal biomarkers of risk through further research.

Breast Cancer and Exposure to Organochlorines in the CECILE Study: Associations with Plasma Levels Measured at the Time of Diagnosis and Estimated during Adolescence.

Bachelet et al,

2019

Int J Env Res Public Health

A French population-based case-control study (CECILE study) of 695 breast cancer cases and 1,055 controls measured plasma levels of organochlorine compounds (OCs)—p,p’-DDE and PCB153—at the time of diagnosis and used a physiologically-based pharmacokinetic (PBPK) model to estimate PCB153 exposure levels during adolescence (ages 11-20), when breast tissue may be particularly susceptible to hormonal disruption. The study found no clear association between measured OC levels at diagnosis and breast cancer risk overall, though there was a trend toward decreasing breast cancer odds ratios with increasing OC levels in women aged 50 and over; similarly, negative associations were observed between breast cancer and estimated adolescent PCB153 exposure levels. The PBPK modeling revealed that women born after 1960 had the highest estimated PCB153 exposures during adolescence (coinciding with peak environmental contamination), while older women had very low adolescent exposures, yet the unexpected negative associations between OC levels and breast cancer risk remained unexplained and may represent study artifacts. Despite these puzzling findings, the study demonstrates that PBPK models can be valuable tools in epidemiological research for back-estimating exposures during critical developmental windows, which could help address important questions about how early-life environmental exposures influence cancer risk decades later.

Environmental chemicals and breast cancer: An updated review of epidemiological literature informed by biological mechanisms.

Rodgers et al,

2018

Environ Res

A systematic review of 158 studies examining environmental chemicals and breast cancer found the strongest evidence for increased risk from exposures during critical developmental periods (in utero, adolescence, pregnancy) to DDT, dioxins, PFOSA, air pollution, and occupational solvents, with risk estimates ranging from 1.4 to 5 times higher. A landmark 50-year study that captured DDT exposure during windows of breast development showed particularly elevated risks, while research on genetic variations found that women with certain DNA repair gene variants had higher breast cancer risk from PAH (polycyclic aromatic hydrocarbon) exposure. However, most studies failed to assess exposure timing during biologically relevant windows of susceptibility, and many current-use chemicals in consumer products remain inadequately studied, with major challenges including reconstructing decades-old exposures and measuring rapidly metabolized chemicals in complex real-world mixtures.

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